[1]杜万根,谢东宝,陆亚洁,等.白腐真菌共培养脱色降解罗丹明B的研究[J].徐州工程学院学报(自然科学版),2020,(03):84-92.
　DU Wangen,XIE Dongbao,LU Yajie,et al.Decolorization and Degradation of Rhodamine B by Co|culture of White Rot Fungi[J].Journal of Xuzhou Institute of Technology(Natural Sciences Edition),2020,(03):84-92.
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白腐真菌共培养脱色降解罗丹明B的研究()
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《徐州工程学院学报》（自然科学版）[ISSN:1674-358X/CN:32-1789/N]

卷:
期数:
2020年03期
页码:
84-92
栏目:
工程技术
出版日期:
2020-09-30

文章信息/Info

Title:
Decolorization and Degradation of Rhodamine B by Co|culture of White Rot Fungi
文章编号:
1674-358X(2020)03-0084-09
作者:
杜万根; 谢东宝; 陆亚洁; 姜东; 赵世光
(安徽工程大学 生物与化学工程学院,安徽 芜湖241000)
Author(s):
DU Wangen; XIE Dongbao; LU Yajie; JIANG Dong; ZHAO Shiguang
(College of Biology and Chemistry Engineering,Anhui Polytechnic University,Wuhu 241000,China)
关键词:
白腐真菌;  共培养;  罗丹明B;  脱色
Keywords:
white rot fungus;  co|cultivation;  rhodamine B;  decolorization
分类号:
X703
文献标志码:
A
摘要:
以白腐真菌共培养方式对合成染料罗丹明B(RhB)进行脱色降解.首先比较了不同白腐真菌组合的漆酶分泌能力差异,选择灵芝菌|金针菇(U281|I16)为优势组合菌对RhB进行脱色降解.基于单因素实验结果分析,设计响应面实验,获得了U281|I16共培养脱色降解RhB的最佳营养条件:果糖19.9 g/L,豆饼粉3.3 g/L,ABTS 1.5 mmol/L,该条件下RhB脱色率可达到93.4%.吸收光谱分析表明,U281|I16共培养处理RhB过程中包含了细胞分泌漆酶的化学催化脱色及菌体细胞物理吸附的双重脱色机制.
Abstract:
The artificial synthetic dye Rhodamine B(RhB)was decolorized and degraded by co|cultivation of white rot fungi.The co|culturing of Ganoderma lucidum and Flammulina velutipes(U281|I16)was selected to decolorize RhB after comparing the differences of laccase secreting capacity of diverse white rot fungi combinations.On the basis of single|factor experiment analysis,a response surface experiment was designed to obtain the optimal nutritional conditions for RhB decolorization by U281|I16.The RhB decolorization rate was up to 93.4% under the optimal conditions as following:19.9 g/L of fructose,3.3 g/L of soybean cake powder and 1.5 mmol/L of ABTS.Absorption spectrum analysis suggested that the dual mechanisms including chemical catalysis of laccase and physical absorption of cells occurred in the process of RhB treatment by co|cultivation of U281|I16.

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备注/Memo

备注/Memo:
收稿日期:2020-03-20
基金项目:(安徽省高校自然科学研究项目(KJ2019A0143; GXXT|2019|012; B2018|03); 安徽省大学生创新创业训练计划项目(201810363202))
作者简介:杜万根(1996-),男,硕士研究生,主要从事应用微生物学研究.
更新日期/Last Update: 2020-09-30