[1]杜万根,谢东宝,陆亚洁,等.白腐真菌共培养脱色降解罗丹明B的研究[J].徐州工程学院学报(自然科学版),2020,(03):84-92.
 DU Wangen,XIE Dongbao,LU Yajie,et al.Decolorization and Degradation of Rhodamine B by Co|culture of White Rot Fungi[J].Journal of Xuzhou Institute of Technology(Natural Sciences Edition),2020,(03):84-92.
点击复制

白腐真菌共培养脱色降解罗丹明B的研究()
分享到:

《徐州工程学院学报》(自然科学版)[ISSN:1674-358X/CN:32-1789/N]

卷:
期数:
2020年03期
页码:
84-92
栏目:
工程技术
出版日期:
2020-09-30

文章信息/Info

Title:
Decolorization and Degradation of Rhodamine B by Co|culture of White Rot Fungi
文章编号:
1674-358X(2020)03-0084-09
作者:
杜万根谢东宝陆亚洁姜东赵世光
(安徽工程大学 生物与化学工程学院,安徽 芜湖241000)
Author(s):
DU WangenXIE DongbaoLU YajieJIANG DongZHAO Shiguang
(College of Biology and Chemistry Engineering,Anhui Polytechnic University,Wuhu 241000,China)
关键词:
白腐真菌 共培养 罗丹明B 脱色
Keywords:
white rot fungus co|cultivation rhodamine B decolorization
分类号:
X703
文献标志码:
A
摘要:
以白腐真菌共培养方式对合成染料罗丹明B(RhB)进行脱色降解.首先比较了不同白腐真菌组合的漆酶分泌能力差异,选择灵芝菌|金针菇(U281|I16)为优势组合菌对RhB进行脱色降解.基于单因素实验结果分析,设计响应面实验,获得了U281|I16共培养脱色降解RhB的最佳营养条件:果糖19.9 g/L,豆饼粉3.3 g/L,ABTS 1.5 mmol/L,该条件下RhB脱色率可达到93.4%.吸收光谱分析表明,U281|I16共培养处理RhB过程中包含了细胞分泌漆酶的化学催化脱色及菌体细胞物理吸附的双重脱色机制.
Abstract:
The artificial synthetic dye Rhodamine B(RhB)was decolorized and degraded by co|cultivation of white rot fungi.The co|culturing of Ganoderma lucidum and Flammulina velutipes(U281|I16)was selected to decolorize RhB after comparing the differences of laccase secreting capacity of diverse white rot fungi combinations.On the basis of single|factor experiment analysis,a response surface experiment was designed to obtain the optimal nutritional conditions for RhB decolorization by U281|I16.The RhB decolorization rate was up to 93.4% under the optimal conditions as following:19.9 g/L of fructose,3.3 g/L of soybean cake powder and 1.5 mmol/L of ABTS.Absorption spectrum analysis suggested that the dual mechanisms including chemical catalysis of laccase and physical absorption of cells occurred in the process of RhB treatment by co|cultivation of U281|I16.

参考文献/References:

[1] VIJAYKUMAR MH,VEERANAGOUDA Y,NEELAKANTESHWAR K,et al.Decolorization of 1:2 metal complex dye Acid blue 193 by a newly isolated fungus,Cladosporium cladosporioides[J].World Journal of Microbiology & Biotechnology,2006,22(2):157|162.
[2] FORGACS E,CSERHATI T,OROS G.Removal of synthetic dyes from wastewaters:a review [J].Environment International,2004,30(7):953|971.
[3] 王晓青,张龙,刘汉湖,等.粉末活性炭对两种染料的吸附动力学研究[J].徐州工程学院学报(自然科学版),2010,25(3):51|55.
[4] 王炫,李焕焕,张乾,等.蛋白土-Fe2O3非均相Fenton催化剂高效降解罗丹明B染料废水[J].化工进展,2017,36(8):3116|3124.
[5] 张桐,张波,李玉.三种白腐真菌脱色噻嗪染料亚甲基蓝[J].菌物学报,2018,37(9):1243|1250.
[6] WANG N,CHU Y,WU F,et al.Decolorization and Degradation of Congo red by a newly isolated white rot fungus,Ceriporia lacerata,from decayed mulberry branches[J].International Biodeterioration & Biodegradation,2017,117:236|244.
[7] 姜滢滢,朱双双,章苗,等.微生物共培养研究进展[J].中国微生态学杂志,2017,29(2):239|244.
[8] 徐德阳,王莉莉,杜春梅.微生物共培养技术的研究进展[J].微生物学报,2015,55(9):1089|1096.
[9] 郭晓丹娜,郭夏丽.白腐真菌共培养对磺胺二甲基嘧啶降解的影响[J].生物技术通报,2017,33(5):197|202.)
[10] 刘坤,李会宣,李敬.白腐真菌菌株共培养降解玉米秸秆的研究[J].安徽农业科学,2008(4):1327|1329.
[11] 王林,胡玲,郑在,等.酶法水解碎米制取碎米小分子多肽[J].食品工业科技,2013,34(12):203|206.
[12] 林俊芳,刘志明,陈晓阳,等.真菌漆酶的酶活测定方法评价[J].生物加工过程,2009,7(4):1|8.
[13] JÖRG S,WOLFGANG H.Carbon catabolite repression in bacteria [J].Current Opinion in Microbiology,1999,2(2):195|201.
[14] 徐鑫,张国庆,胡渤洋,等.真菌漆酶及其介体系统:来源、机理与应用[J].生物技术进展,2020,10(1):30|39.
[15] ENAYATZAMIR K,ALIKHANI H A,RODRÍGUEZ C S.Simultaneous production of laccase and decolouration of the diazo dye Reactive Black 5 in a fixed|bed bioreactor [J].Journal of Hazardous Materials,2009,164(1):296|300.
[16] 陶国翔,李爱秀,张敏,等.漆酶与ABTS间的结合模式与作用机制研究[J].化学与生物工程,2017,34(11):23|26.
[17] 田东凡,王玉如,宋薇,等.UV/PMS降解水中罗丹明B的动力学及反应机理[J].环境科学学报,2018,38(5):1868|1876.

相似文献/References:

[1]梁峙,李勇,裴宗平.生物絮凝剂在处理洗煤废水中的应用[J].徐州工程学院学报(自然科学版),2009,(1):42.[doi:10.3969/j.issn.1674-358X.2009.01.009]
 LIANG Zhi,LI Yong,PEI Zong-ping.The Optimization of the Conditions in Processing Slime Water of High Concentration and Fine Grain with Bio-flocculant[J].Journal of Xuzhou Institute of Technology(Natural Sciences Edition),2009,(03):42.[doi:10.3969/j.issn.1674-358X.2009.01.009]

备注/Memo

备注/Memo:
收稿日期:2020-03-20
基金项目:(安徽省高校自然科学研究项目(KJ2019A0143; GXXT|2019|012; B2018|03); 安徽省大学生创新创业训练计划项目(201810363202))
作者简介:杜万根(1996-),男,硕士研究生,主要从事应用微生物学研究.
更新日期/Last Update: 2020-09-30