[1]韩伟,邓祥.酶法、β-环糊精协同超声提取茶多酚及抗氧化活性[J].徐州工程学院学报(自然科学版),2019,(03):34-42.
 HAN Wei,DENG Xiang.Extraction of Tea Polyphenols and Antioxidant Activity by Enzymatic and β-Cyclodextrin Combined with Ultrasound[J].Journal of Xuzhou Institute of Technology(Natural Sciences Edition),2019,(03):34-42.
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酶法、β-环糊精协同超声提取茶多酚及抗氧化活性()
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《徐州工程学院学报》(自然科学版)[ISSN:1674-358X/CN:32-1789/N]

卷:
期数:
2019年03期
页码:
34-42
栏目:
教授论坛
出版日期:
2019-09-15

文章信息/Info

Title:
Extraction of Tea Polyphenols and Antioxidant Activity by Enzymatic and β-Cyclodextrin Combined with Ultrasound
文章编号:
1674-358X(2019)03-0034-09
作者:
韩伟邓祥
(华东理工大学 药学院 制药工程与过程化学教育部工程研究中心; 上海市新药设计重点实 验室,上海200237)
Author(s):
HAN WeiDENG Xiang
(Engineering Research Centre of Pharmaceutical Process Chemistry,Ministry of Education; Shanghai Key Laboratory of New Drug Design; School of Pharmacy,East China University of Science and Technology,Shanghai 200237,China)
关键词:
酶法 β-环糊精 超声 协同提取 抗氧化活性
Keywords:
enzyme β-cyclodextrin ultrasound collaborative extraction antioxidant activity
分类号:
R284.2
文献标志码:
A
摘要:
以炒青绿茶为原料,采用酶法、β-环糊精协同超声提取茶多酚,通过单因素方差试 验初步确定提取过程的主要影响因素,再设计中心组合试验,进一步优化得到最佳工艺参数: 纤维素酶用量为0.57 mL,β-环糊精0.69 g,液料比37,超声时间60 min,超声温度60 ℃,此时 茶多酚提取得率达13.87%,比传统水提取2次的得率高6.86%,所得茶多酚具有较好的抗氧化活 性,相同质量浓度下茶多酚总还原性、ABTS+·清除能力和·OH清除能力明显高 于Vc,DPPH·清除能力与Vc相当.
Abstract:
Tea polyphenols were extracted from stir-fried green tea by enzymatic method and ultrasound combined with β-cyclodextrin.The main factors affecting the extraction process were determined preliminarily by single factor variance test,and then the central combination test was designed to further optimize the process parameters:the dosage of cellulase was 0.57 mL,the dosage of β- cyclodextrin was 0.69 g,and the liquid content was 0.57 mL.The yield of tea polyphenols reached 13.87% when the ratio of materials was 37,the ultrasonic time was 60 min,and the ultrasonic temperature was 60 ℃.The yield of tea polyphenols was 6.86% higher than that of traditional water extraction twice.The tea polyphenols obtained had better antioxidant activity.The total reducing power,ABTS+·clearing ability and ·OH scavenging ability of tea polyphenols were significantly higher than Vc at the same mass concentration,and DPPH·clearing ability was comparable to Vc.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2019-03-30 基金项目:国家自然科学基金项目(20006003) 作者简介:韩伟(1968-),男,教授,博士,博士生导师,主要从事天然产物的提取、分离及功能 研究.
更新日期/Last Update: 2019-09-15