[1]舒旭晨,杜万根,姜 东,等.混菌发酵石斛酵素及其抗氧化活性研究[J].徐州工程学院学报(自然科学版),2019,(2):63-70.
 SHU Xuchen,DU Wangen,JIANG Dong,et al.Mixed Fermentation of Dendrobium Enzyme and its Antioxidant Activity[J].Journal of Xuzhou Institute of Technology(Natural Sciences Edition),2019,(2):63-70.
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混菌发酵石斛酵素及其抗氧化活性研究()
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《徐州工程学院学报》(自然科学版)[ISSN:1674-358X/CN:32-1789/N]

卷:
期数:
2019年第2期
页码:
63-70
栏目:
应用基础研究
出版日期:
2019-05-30

文章信息/Info

Title:
Mixed Fermentation of Dendrobium Enzyme and its Antioxidant Activity
文章编号:
1674-358X(2019)02-0063-08
作者:
舒旭晨杜万根姜 东高 越魏 明赵世光
(安徽工程大学 生物与化学工程学院,安徽 芜湖 241000)
Author(s):
SHU XuchenDU WangenJIANG DongGAO YueWEI MingZHAO Shiguang
(College of Biology and Chemistry Engineering,Anhui Polytechnic University,Wuhu 241000,China)
关键词:
石斛酵素 抗氧化活性 响应面法 条件优化
Keywords:
Dendrobium enzyme antioxidant activity response surface method condition optimization
分类号:
TQ920.1
文献标志码:
A
摘要:
以铁皮石斛鲜条为原料,以酵母菌、乳酸菌为混菌发酵剂,对石斛酵素发酵条件进行优化研究.以单因素实验法分别考察碳源、菌种配比、温度、料液比、接种量等因素对石斛酵素发酵的影响.获取较优因素后,以羟基自由基清除率为响应值,采用数理统计学方法对蔗糖质量分数、菌种配比、发酵温度三因素进行优化.结果表明:在蔗糖质量分数8.12%、菌种配比1:2(酵母菌:乳酸菌)、发酵温度31 ℃条件下,制备的石斛酵素羟基自由基清除率达到34.79%,较优化前提升了108.45%.
Abstract:
The Dendrobium officinale enzyme is fermented by mixed cultivation of yeast and lactic acid bacteria.Single factor experiment was used to study the effect of carbon source,strain ratio,temperature,solid-liquid ratio and inoculation amount on the fermentation of Dendrobium officinale enzyme.Based on the results of single factor experiment,the sucrose content,strain ratio and fermentation temperature were optimized by mathematical statistics when targeting on hydroxyl radical scavenging rate.The results showed that the optimum conditions were 8.12% of sucrose addition,1:2 of strain ratio and 31 ℃ of fermentation temperature,under which the hydroxyl radical scavenging rate of Dendrobium officinale enzyme was up to 34.79% and increase by 108.45%.

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备注/Memo

备注/Memo:
收稿日期:2019-03-10 基金项目:安徽省高校自然科学研究项目(KJ2017A101); 安徽省大学生创新创业训练计划项目(201610363212,201710363199,201810363202); 安徽工程大学宣城产业研究项目(B2018-03) 作者简介:舒旭晨(1988-),男,硕士研究生,主要从事食品资源开发与利用研究.
更新日期/Last Update: 2019-05-30