[1]李同祥,李文,孙会刚,等.农药2,4-滴丁酯与敌敌畏降解菌株的筛选及性能研究[J].徐州工程学院学报(自然科学版),2017,(2):30-35.
 LI Tongxiang,LI Wen,SUN Huigang,et al.Identification and Degradation of a Bacterium LT1 in Degrading Pesticide 2,4-D Butyl Ester and DDVP[J].Journal of Xuzhou Institute of Technology(Natural Sciences Edition),2017,(2):30-35.
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农药2,4-滴丁酯与敌敌畏降解菌株的筛选及性能研究()
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《徐州工程学院学报》(自然科学版)[ISSN:1674-358X/CN:32-1789/N]

卷:
期数:
2017年第2期
页码:
30-35
栏目:
专家特稿
出版日期:
2017-04-28

文章信息/Info

Title:
Identification and Degradation of a Bacterium LT1 in Degrading Pesticide 2,4-D Butyl Ester and DDVP
文章编号:
1674-358X(2017)02-0030-06
作者:
李同祥李文孙会刚侯进慧王陶黄天姿汤薇陈尚龙
徐州工程学院 食品工程学院,江苏 徐州 221018
Author(s):
LI Tongxiang LI Wen SUN Huigang HOU Jinhui WANG Tao HUANG Tianzi TANG Wei CHEN Shanglong
College of Food Engineering, Xuzhou Institute of Technology, Xuzhou, 221018, China
关键词:
24-滴丁酯 敌敌畏 鉴定 降解特性 基因定位
Keywords:
24-D butyl ester DDVP identification degradation characteristics gene location
分类号:
X592
文献标志码:
A
摘要:
通过以单一农药为碳源的选择性培养基驯化,从农药厂污水处理池活性污泥中筛选出一株能够降解有机氯农药2,4-滴丁酯和有机磷农药敌敌畏的菌株,根据菌株的形态、生理生化特征、16S rRNA序列分析,确定为假单胞菌(Pseudomonas sp.LT1 ).研究表明,该菌株最适生长温度为30 ℃,最适生长pH为7.2,对2,4-滴丁酯和敌敌畏的理想降解起始质量浓度为300 mg/L; 在以2,4-滴丁酯和敌敌畏为唯一碳源的无机盐培养基条件下其降解率分别为93.82%和95.65%,对土壤中的2,4-滴丁酯和敌敌畏的降解率分别为96.20%和97.06%.菌株Pseudomonas sp.LT1对2,4-滴丁酯和敌敌畏的降解酶基因位于基因组上.
Abstract:
The sample was taken from active sludge of pesticide plant treatment tank,and a degradation strains were isolated from it with selective medium with a single pesticide as a carbon source.The degradation strains has a capable of degrading 2,4-D butyl ester and DDVP efficiently.It was initially identified asPseudomonas sp.(Named asPseudomonas sp.LT1 )with the colony observation,physiological and biochemical features and 16S rRNA sequence.The research shows that the optimum growth temperature of it was 30 ℃ and the optimum growth pH was 7.2,the initial concentration of 2,4-D butyl ester and DDVP was 300 mg/L respectively.Under the condition of inorganic salt medium including 2,4-D butyl ester and DDVP as the sole carbon source,The rates of the strains to degrade 2,4-D butyl ester and DDVP were 93.82%和95.65% respectively and the degradation rates of 2,4-D butyl ester and DDVP were 93.82%和95.65% in the soil, respectively.Then the degrading gene of strain LT1 was studied preliminarily,the results showed that the degrading gene of 2,4-D butyl ester and DDVP in strain LT1 was located on its genome.

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备注/Memo

备注/Memo:
收稿日期:2017-03-03 基金项目:国家自然科学基金项目(31270577); 江苏省高校自然科学研究重大项目(16KJA210001) 作者简介:李同祥(1966-),男,教授,博士,主要从农药污染生态修复和污染物生物芯片检测技术研究.
更新日期/Last Update: 1900-01-01